Dear colleagues,

The study of various proteins and enzymes has become quite widespread in recent years. Protein engineering is a method that is used both to solve fundamental scientific problems, for example, cloning the genes of certain proteins, and also biotechnological tasks, for example - to obtain proteins with required characteristics. With the help of protein engineering, it’s possible to simplify the protein purification method by adding a special tag, which is then used to purify the protein, increase the solubility of the protein product, as well as increase stability, specific activity, change the pH and temperature optimum, and substrate specificity. Also, with the help of protein engineering, it is possible to create completely new proteins and enzymes that did not exist before, but which can play an important role in biotechnological processes. Currently, thanks to the development of computer methods for modeling protein structures, it has become possible to predict the properties of proteins, conduct a detailed analysis of protein structures, and build theoretical models of the relationships between changes in the structure and properties of proteins. Therefore, protein engineering is now experiencing a new round of development and is becoming interesting to a wide range of scientists.

In this section we can collect articles devoted to various methods and approaches to protein engineering of proteins and enzymes, scientists can share their ideas concerning obtaining novel enzymes with required characteristics for different biotechnological purposes. 

cloning; site-directed mutagenesis; expression; enzyme properties; stability of proteins; protein modelling; protein structure-function relationship