Effect of electrospun SF/CS composite fiber scaffold on cell proliferation and osteogenic differentiation of hBMSCs in vitro

Abstract

Objective: Using electrospinning to preparesilk fibroin/chitosan (SF/CS) nanofiber membrane scaffolds, and then evaluating its properties and effects on proliferation and osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs) . Methods: The regenerated silk fibroin (SF) and chitosan (CS) were dissolved in the mixed solvent system of trifluoroacetic acid and dichloromethane by mass ratio (1 : 0, 1 : 1) . The structure and properties of the electrospun films were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and thermal gravity/differential thermal gravity analysis (TG/DTG). Cells in the experimental group were inoculated on the surface of SF and SF/CS membrane respectively. Cells in the control group were directly inoculated in culture dish. hBMSCs were used in each group to induce osteogenesis. CCK-8 was used to study the growth and proliferation of cells. Energy dispersive spectrometer (EDS) and alizarin red staining (ARS) were used to detect the ability of osteogenesis and mineralization. Results: Compared with SF scaffolds, SF/CS scaffolds had more uniform fiber diameter (SEM) and more stable conformation (FTIR) ; TG/DTG results showed that SF scaffolds had more thermal stability. CCK-8 showed that compared with the control group, there was no significant difference in proliferation of hBMSCs between SF and SF/CS groups when co-cultured for 5 and 7 days (P>0.05) . After 21 days of culture, elemental analysis indicated that the SF/CS group had higher calcium content. Compared with the control group and SF group, calcified nodules of hBMSCs in SF/CS group were significantly increased and staining was deep. Conclusions: Electrospinning SF/CS nanofibers scaffolds have good biocompatibility and can promote osteogenic differentiation of hBMSCs.